Morphology of Tibiofemoral Joint in Rats with Experimental Osteoarthritis: 3D-reconstruction, Based on the Technology of High-Precision Grinding

The aim was to study morphological characteristics of rats’ tibiofemoral joint articular cartilage using the method of multiple high-precision grinding in the simulation of experimental osteoarthritis. Materials and methods. An experimental study was carried out on 16 mature white male Wistar rats. Modeling of osteoarthritis was carried out by inserting into tibiofemoral joints of 10 rats the suspension of sterile medical talc with saline solution in the ratio 1: 5. The animals were withdrawn from the experiment after 6 and 12 weeks by an overdose of the drug "Bioveta" (Czech Republic) in a dose of 200 mg / kg of body weight. The technology of high-precision layer-by-layer sanding in combination with digital survey of thin sections and software for creating a virtual model of the original object were used in creating 3D-reconstructions. A quantitative comparative study was conducted using the "ImageJ" program. Results. 3D-reconstruction of joints devoid of spatial and color distortions were obtained, which allow to obtain both correct anatomical sections in given planes, and normals to surfaces for exact linear measurements. Quantitative indices describing the structure of the articular cartilage are described in the norm and with a decrease in the lubricative properties of the synovial fluid. The thickness of the articular cartilage was reduced by the 12th week of the experiment, while the surface factor slightly increased, which indicated partial damage to the joint surface. The obtained RGB-profiles of digitized images of articular cartilage of the rat tibiofemoral joint allowed to reveal on 3D-reconstructions new early signs of articular cartilage degeneration during experimental reproduction of osteoarthritis.

суставной хрящ, морфология, остеоартроз, математические методы в биологии, 3D-реконструкции, articular cartilage, morphology, osteoarthrosis, mathematical methods in biology, 3D reconstruction

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