Migration Flow Dynamics of Primordial Germ Cells in the Development of Female Gonads Anlagen

Aim: to study activity and migration patterns of primordial germ cells (PGC) in a human embryo in the development of female gonads anlagen.

Material and methods. The objects of research (human embryos) were obtained as a result of medical abortions in healthy women at the medical institutions of Tyumen. Each of the pregnant women gave their informed consent allowing the use of abortion material in scientific research. The volume of the material was 127 human embryos from the 12th to 23d Carnegie stage (CS). The number of embryos at each stage was from 4 till 20 embryos. Material was formalin-fixed and paraffin-embedded. Section staining was performed with Mayer’s hematoxylin and with eosin. Schiff-positive substrates according to McManus method were revealed histochemically. Light microscopy equipment such as microscope, digital camera Canon EOS 5 D and computer were used to reveal migration patterns of primordial germ cells (PGC). Statistical analysis was carried out with the help of computer programmes «StatSoft Statistica 12» and «IBM SPSS Statistics Standart» using Student’s test.

Results. Observations on migration flow of primordial germ cells revealed two significant parameters: periodicity in migration activity and local fixation on the way of movement in the embryo. The most important indices of primordial germ cells migration chronovector correspond to the 12th, the 16th and the 21st Carnegie stage (CS). The patterns of primordial germ cells fixation - discrete and cluster - were revealed.

Conclusion. In fetal period in human embryo multiple loci of female gonads anlagen develop. The most favorable areas are mesonephric-gonadal complexes and adrenal glands.

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