Features of Macro-Microscopic Morphology of the Human Large Intestine Glands with a Predominant Vegetarian Type of Diet
https://doi.org/10.18499/2225-7357-2019-8-4-9-14
Abstract
The aim of investigation is to obtain data about the macro- and microscopic structure, as well as the cellular composition of the human large intestine glands from people who long adhere to the predominant vegetarian type of food.
Material and methods. We conducted a comparative study of structural and quantitative indicators of the glandular apparatus of the large intestine of the corpses of four people 60–89 years of age, who had long adhered to a predominantly vegetarian diet. Microscopic observation was carried out on histological preparations stained with methylene blue, hematoxylin-eosin, van Gieson, Grimalius, alcyan blue. For statistical data processing methods of parametric statistics based on the program Statistica 6.0 were used. Statistical data processing included calculation of arithmetic averages and their errors.
Results. The analysis of the qualitative assessment of the cellular composition of the epithelium and morphometric measurements of the size parameters of the intestinal glands in a long vegetarian diet revealed a significant increase in both the number and area of the intestinal glands: with a maximum for the caecum and sigmoid intestine in 126% and 179%, respectively. There was a statistically significant 5% increase in the proportion of goblet cells in glandular tissue and a corresponding increase in the volume of mucus in the consumption of plant foods. The proportion of undifferentiated (cambial) cells decreased slightly (about 3.5%). Indicators of the glands of the proximal 2/3 of length of the large intestine showed a significant proximo-distal gradient: there was a smooth decrease in both the number of epithelial cells and their morphometric characteristics.
About the Authors
N. T. AlexeevaRussian Federation
Nataliya Alexeeva.
ul. Studencheskaya, 10, Voronezh, 394036.
S. V. Klochkova
Russian Federation
Moscow.
E. A. Rozhkova
Russian Federation
Moscow.
D. B. Nikityuk
Russian Federation
Moscow.
References
1. Bykova AP, Kozlova IV. Clinical-endoscopic and morphological features of the colon in chronic pancreatitis. Eksperimental’naya i Klinicheskaya Gastroenterologiya. 2017; 139(3):22–7 (in Russian).
2. Kaprelyants LV, Buzhylov NG. Wheat cuts as sources of useful human consumpers. Aktual'nye nauchnye issledovaniya v sovremennom mire. 2019; 4-2(48):14–9 (in Russian).
3. Korzhevskii DE, Gilyarov AV. Osnovy gistologicheskoi tekhniki. Saint-Petersburg: SpetsLit; 2010. 95 (in Russian).
4. Nikityuk DB, Kolesnikov LL, Shadlinskii VB, Bazhenov DV, Alekseeva NT, Klochkova SV. Mnogokletochnye zhelezy stenok pishchevaritel'noi i dykhatel'noi sistem (voprosy funktsional'noi morfologii). Voronezh: Nauchnaya kniga; 2017. 278 (in Russian).
5. Podbeltsev DA, Nikityk DB, Posdnyakov AL. Influence of prebiotics on morphologic structure of the mucouse membrane of intestinum crassum of rats. Problems of Nutrition. 2006; 75(2):26–9 (in Russian).
6. Tamova MYu, Shamkova NT, Tutel'yan VA. Organizatsiya pitaniya detei rannego i doshkol'nogo vozrasta v doshkol'noi obrazovatel'noi organizatsii. Moscow: DeLi plyus; 2019. 157 (in Russian).
7. Tutel'yan VA, Gapparov MMG, Kaganov BS, Sharafetdinov KhKh. Lechebnoe pitanie: sovremennye podkhody k standartizatsii dietoterapii. Moscow: Dinastiya; 2010. 304] (in Russian).
8. Fillips SF. Fiziologiya i patofiziologiya zheludochno-kishechnogo trakta. Moscow: Meditsina; 1989: 340–55] (in Russian).
9. Yatskovskii AN, Boronikhina TV. Vliyanie ratsiona s izbytkom kletchatki na morfofunktsional'noe sostoyanie duodenal'nykh zhelez krys. Arkhiv anatomii. 1987. 88 (11):87–92 (in Russian).
10. Cheng L, Kong C, Walvoort MTC, Faas MM, de Modulate Goblet Cells Under Homeostatic, Proinflammatory Conditions and Under ER-Stress. Mol Nutr Food Res. 2019 Dec 4:e1900976. doi: 10.1002/mnfr.201900976.
11. Doughty MJ. Multiple count sampling of goblet cells in microscope high-power fields using conjunctival impression cytology. Clin Exp Optom. 2019 Dec 3. doi: 10.1111/cxo.13007.
12. Ficsor L, Varga VS, Tagscherer A, Tulassay Z, Molnar B. Automated classification of inflammation in colon histological sections based on digital microscopy and advanced image analysis. Cytometry Part A. 2008 Mar;73A(3):230–7. doi: 10.1002/cyto.a.20527
13. Kleessen B, Hartmann L, Blaut M. Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats. British Journal of Nutrition. 2003 May;89(5):597–606. doi: 10.1079/bjn2002827
14. Pereira RT, Nebo C, de Paula Naves L et all. Distribution of goblet and endocrine cells in the intestine: A comparative study in Amazonian freshwater Tambaqui and hybrid catfish. J Morphol. 2020 Jan;281(1):55-67. doi: 10.1002/jmor.21079.
15. Treveil A, Sudhakar P, Matthews ZJ et al. Regulatory network analysis of Paneth cell and goblet cell enriched gut organoids using transcriptomics approaches. Mol Omics. 2019 Dec 10. doi: 10.1039/c9mo00130a.
16. Weijun T, Teng Z. Probiotics inhibit immune fluctuation in the intestinal mucous layer in rats. Surgery Today. 2015 Feb 11;45(12):1553–9. doi: 10.1007/s00595-015-1123-5
Review
For citations:
Alexeeva N.T., Klochkova S.V., Rozhkova E.A., Nikityuk D.B. Features of Macro-Microscopic Morphology of the Human Large Intestine Glands with a Predominant Vegetarian Type of Diet. Journal of Anatomy and Histopathology. 2019;8(4):9-14. (In Russ.) https://doi.org/10.18499/2225-7357-2019-8-4-9-14