Histochemical approaches to the evaluation of the participation of mast cells in the regulation of the fibrous component of the intercellular matrix of skin connective tissue
https://doi.org/10.18499/2225-7357-2018-7-3-100-112
Abstract
The article presents the data of study of the effectiveness of various combinations of histochemical staining to determine the co-location of mast cells with a fibrous component of the connective tissue of Wistar line laboratory rats skin. The best results are obtained with combination protocols such as metachromatically detection of mast cells in combination with the impregnation with silver or picrofuchsin, brilliant green coloring with the methodology of Van Gieson or picrofuchsin, and aniline blue neutral red. The proposed variants of histochemical protocols can be used to analyze the participation of mast cells in the remodeling of the cell matrix of tissue microenvironment in the development of adaptive and pathological processes. The obtained results contribute to the expansion of currently available scientific ideas about the laws of the process of fibrillogenesis in the extracellular matrix and provide new data on the targeted participation of the components of the mast cell secretome in the molecular mechanisms of fiber formation.
About the Author
D. A. Atyakshin
Voronezh N. N. Burdenko State Medical University, Voronezh, Russia
Russian Federation
Russian Federation
References
1. Атякшин Д. А., Бухвалов И. Б., Тиманн М. Гистохимия ферментов. Воронеж; 2017. 120.
2. Елисеев В. Г. Соединительная ткань: гистофизиологические очерки. М.: Медгиз; 1961. 461.
3. Коржевский Д. Э. Морфологическая диагностика. Подготовка материала для гистологического исследования и электронной микроскопии: руководство. СПб.; 2013. 127.
4. Лили Р. Патогистологическая техника и практическая гистохимия: пер. с англ. чл.-корр. АМН Португалова В.В., ред. М.: Мир; 1969. 845.
5. Омельяненко Н. П., Слуцкий Л. И. Соединительная ткань (гистофизиология и биохимия). Акад. РАН и РАМН Миронов С.П., ред. М.: Известия; 2009. 1: 380.
6. Пирс Э. Гистохимия. Теоретическая и практическая: пер. с англ. М.: Изд-во иностранной литературы, 1962. 962.
7. Саркисов Д. С., Перов Ю. Л. Микроскопическая техника: руководство. М.: Медицина; 1996. 544.
8. Серов В. В., Шехтер А. Б. Соединительная ткань (функциональная морфология и общая патология). М.: Медицина; 1981. 312.
9. Фукс Б. Б., Фукс Б. И. Очерки морфологии и гистохимии соединительной ткани. Л.: Медицина; 1968. 216.
10. Atiakshin D., Buchwalow I., Samoilova V., Tiemann M. Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol. 2018; 149(5): 461-477.
11. Atiakshin D., Samoilova V., Buchwalow I., Boecker W., Tiemann M. Characterization of mast cell populations using different methods for their identification. Histochem Cell Biol. 2017; 147(6): 683-694.
12. Bancelin S., Decenciere E., Machairas V., Albert C., Coradin T., Schanne-Klein M. C., Aime C. Fibrillogenesis from nanosurfaces: multiphoton imaging and stereological analysis of collagen 3D self-assembly dynamics. Soft Matter. 2014; 10(35): 6651-6657.
13. Birk D. E., Fitch J. M., Babiarz J. P., Doane K. J., Linsenmayer T. F. Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter. J CellSci. 1990; 95(4): 649-657.
14. Bornstein P., Sage E. H. Matricellular proteins: extracellular modulators of cell function. Current opinion in cell biology. 2002; 14(5): 608-616.
15. Buchwalow I., Boecker W., Tiemann M. The contribution of Paul Ehrlich to histochemistry: a tribute on the occasion of the centenary of his death. Virchows Arch. 2015; 466: 111-116.
16. Byers P. H. Collagens: building blocks at the end of the development line. Clinical genetics. 2000; 58(4): 270-279.
17. Chen H., Xu Y., Yang G., Zhang Q., Huang X., Yu L., Dong X. Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF-β1/Smads signaling pathway. ExpTher Med. 2017; 14(5): 4438-4442.
18. Conti P., Caraffa A., Mastrangelo F., Tettamanti L., Ronconi G., Frydas I., Kritas S. K., Theoharides T. C. Critical role of inflammatory mast cell in fibrosis: Potential therapeutic effect of IL-37. Cell Prolif. 2018: e12475. doi: 10.1111/cpr.12475.
19. Dwyer D. F., Barrett N. A., Austen K. F. Immunological Genome Project C: Expression profiling of constitutive mast cells reveals a unique identity within the immune system. Nat Immunol. 2016; 17: 878-887
20. Espinosa E., Valitutti S. New roles and controls of mast cells. Curr Opin Immunol. 2018; 50: 39-47.
21. Fibrosis: Methods and Protocols. Edited by Laure Rittie. 2017. 530.
22. Frossi B., Mion F., Sibilano R., Danelli L., Pucillo C. E. M. Is it time for a new classification of mast cells? What do we know about mast cell heterogeneity? Immunol Rev. 2018; 282(1): 35-46.
23. Galli S. J., Tsai M. Mast cells: versatile regulators of inflammation, tissue remodeling, host defense and homeostasis. J Dermatol Sci. 2008; 49: 7-19.
24. Ghazanfari S., Khademhosseini A., Smit T. H. Mechanisms of lamellar collagen formation in connective tissues. Biomaterials. 2016; 97: 74-84.
25. Harris J. R., Lewis R. J. The collagen type I segment long spacing (SLS) and fibrillar forms: Formation by ATP and sulphonated diazo dyes. Micron. 2016; 86: 36-47.
26. Hitchcock A. M., Yates K. E., Costello C. E., Zaia J. Comparative glycomics of connective tissue glycosaminoglycans. Proteomics. 2008; 8(7): 1384-1397.
27. Hügle T. Beyond allergy: the role of mast cells in fibrosis. Swiss Med Wkly. 2014; 144: w13999. doi: 10.4414/smw.2014.13999.
28. Ina K., Kitamura H., Tatsukawa S., Miyazaki T., Abe H., Fujikura Y. Intracellular formation of collagen microfibrils in granulation tissue. ExpMolPathol. 2005; 79(3): 244-248.
29. Kulke M., Geist N., Friedrichs W., Langel W. Molecular dynamics simulations on networks of heparin and collagen. Proteins. 2017; 85(6): 1119-1130.
30. Loerakker S., Obbink-Huizer C., Baaijens F. P. A physically motivated constitutive model for cell-mediated compaction and collagen remodeling in soft tissues. Biomech Model Mechanobiol. 2014; 13(5): 985-1001.
31. Muldashev E. R., Muslimov S. A., Musina L. A., Nigmatullin R. T., Lebedeva A. I., Shangina O. R., Khasanov R. A. The role of macrophages in the tissues regeneration stimulated by the biomaterials. Cell Tissue Bank. 2005; 6(2): 99-107.
32. Olivera A., Beaven M. A., Metcalfe D. D. Mast cells signal their importance in health and disease. J Allergy ClinImmunol. 2018; 142(2): 381-393.
33. Overed-Sayer C., Rapley L., Mustelin T., Clarke D. L. Are mast cells instrumental for fibrotic diseases? Front Pharmacol. 2014; 4: 174.
34. Pincha N., Hajam E. Y., Badarinath K., Batta S. P. R., Masudi T., Dey R., Andreasen P., Kawakami T., Samuel R., George R., Danda D., Jacob P. M., Jamora C. PAI1 mediates fibroblast-mast cell interactions in skin fibrosis. J Clin Invest. 2018; 128(5): 1807-1819. doi: 10.1172/JCI99088.
35. Redegeld F. A., Yu Y., Kumari S., Charles N., Blank U. Non-IgE mediated mast cell activation. Immunol Rev. 2018; 282(1): 87-113.
36. Robida P. A., Puzzovio P. G., Pahima H., Levi-Schaffer F., Bochner B. S. Human eosinophils and mast cells: Birds of a feather flock together. Immunol Rev. 2018; 282(1): 151-167.
37. Ronnberg E., Melo F. R., Pejler G. Mast cell proteoglycans. J Histochem Cytochem. 2012; 60: 950-962
38. Wernersson S., Pejler G. Mast cell secretory granules: armed for battle. Nat Rev Immunol. 2014; 14: 478-494.
39. Wu K., Li G. Investigation of the Lag Phase of Collagen Fibrillogenesis Using Fluorescence Anisotropy. ApplSpectrosc. 2015; 69(10): 1121-1128.
Review
For citations:
Atyakshin D.A. Histochemical approaches to the evaluation of the participation of mast cells in the regulation of the fibrous component of the intercellular matrix of skin connective tissue. Journal of Anatomy and Histopathology. 2018;7(3):100-112. (In Russ.) https://doi.org/10.18499/2225-7357-2018-7-3-100-112
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