Assessment of decidual macrophages population in patients with non-developing pregnancy

Abstract

The relevance of the search for new pathogenetic mechanisms of non-developing pregnancy is due to the high incidence of the disease and the lack of a downward trend, despite the current reabilitation measures. The purpose of the study: to substantiate approaches оf rehabilitation therapy patients with non-developing pregnancy, based on the data on the quantitative and qualitative composition of macrophages in their decidual tissue. Material and methods. The decidual tissue of patients with non-developing pregnancy and patients who terminated pregnancy at their own will was studied. Macrophages were determined using mouse monoclonal antibodies (Anti-CD68 antibody [KP1], #ab955, dilution 1:200) and rabbit monoclonal antibodies (Anti-CD163 antibody [EPR19518], #ab182422, dilution 1:500) using multiple immunomarkation technique. Results. In missed miscarriage, the number of macrophages is increased twice as compared with physiological course of gestation. Changes in the ratio of macrophages M1 and M2 in the direction of increasing the number of the first. The maximum number of CD68+ cells, as well as macrophages with simultaneous expression of differentiation factors CD68 and CD163 is determined in non-developing pregnancy accompanied by deciduitis. Conclusion. Decidual macrophages participate in the pathogenesis of non-developing pregnancy by increasing their number and changing the polarization vector along the classical pathway of activation, characterized by the active production of рro-inflammatory cytokines and cytotoxic molecules by cells, participation in the implementation of t-helper immune reactions of type 1. Pathogenetically justified the appointment of drugs that can reprogram the macrophage response in patients who have undergone non-developing pregnancy as part of complex rehabilitation therapy.