Histochemical changes in skin burn wound regeneration after application of titanium nickelide alloy supernatant

Objective : to determine the content and distribution in the tissues of the skin of various glycosaminoglycans (GAGs) against the background of the local action of the supernatant of the titanium nickelide alloy, to reveal the positive effect of the substrate on the dynamics of the formation of the regenerative process. Material and methods. The study was carried out on 25 Syrian hamsters (Mesocricetus auratus, Waterhouse, 1839). In stages 1, 3, 7, 14, 21 and 28 days, an overdose of sevoran anesthesia followed by decapitation was used to take the skin flap. The material was processed and poured into paraffin according to the standard conventional procedure. Histological sections were stained with alcian blue with a pH of 1.0 and 2.5, according to Tenzer-Unna. To determine the content of nickel and titanium cations, the electron-probe analysis method applied to the skin grafts taken in all experimental groups was used. Results. the increased accumulation of GAG in the dermis of the control group of animals occurred for a period of 21 days, while in the experiment their maximum was reached already by 14 days. Variations in GAG content in the epidermis and derivatives are associated with a change in the proliferative and biosynthetic activity of the cells of these structures at different periods of the experiment. Given the negative charge of sulfated aminoglycans and the hydrophilic base of the supernatant, penetration of the base metal cations of the supernatant into damaged tissues was detected by scanning microscopy. Conclusions. The reparative processes with the supernatant of the titanium nickelide alloy are more intensive, due to the limitation of the destructive process within the epidermis and the dermis, the reduction of purulent necrotic complications in the healing process, the interaction of nickel and titanium anions and cations in mineral and water metabolism. This ensures an earlier occurrence and uniform maturation of the young connective tissue, epithelization with normalization of cell proliferation and differentiation processes.

кожа, термический ожог, никелид титан, супернатант, гликозаминогликаны, коллаген, skin, thermal burn, titanium nickelide, supernatant, glycosaminoglycans, collagen

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