Genetically-caused tumor formation in lungs of mice of line Af under influence of radiation and chemical factors, and at aging

Abstract

Experimental models of tumor formation using laboratory mice are most often used in the study of mechanisms of carcinogenesis. The line of Af mice is characterized by developing spontaneous tumors in intact animals during development. The aim of the research was to study chemical- and radio-induced carcinogenesis in lungs of mice line Af in vivo. Material and methods. The experiments were performed on mice line Af. Single exposure of irradiation of mice was performed in a dose of 1 Gy. The inhalation effect of sulfur dioxide and ammonia was carried out an hour once. The concentrations of gases in the chamber were 1 mg/m³ and 5 mg/m³. The frequency of tumor formation in the lungs of mice of this line was also analyzed at 12 and 16 months of age. The incidence of tumors (%) and the number of tumors per mouse were evaluated, histological analysis of the detected tumors was performed. Results. In the groups subjected to radiation or inhalation exposure, the proportion of animals with lung adenomas were significantly increased compared to the control group. Neoplasms in the lungs of mice in all studied groups had a similar histological structure and were classified as highly differentiated adenocarcinoma. Conclusions. The obtained results suggest that the chemical inhalation and radiation (1 Gy) effect in this experiment was the cause of genetically determined formation of tumors at an earlier age.