MORPHOFUNCTIONAL CHARACTERISTIC OF PARAFOLLICULAR CELLS OF THYROID GLAND IN DOGS AT VARIOUS KINDS OF PHYSICAL ACTIVITY

The aim was to study the influence of physical exertion on intrathyroid humoral regulators of the thyroid gland (TG) – parafollicular cells (C-thyrocytes).

Material and methods. The animals of the intact group (12 male dogs) and two experimental groups (11 dogs in each) were studied. As their motor activity (running on the treadmill tape) various types of loads were received: training (before long duration of run – 1^st  group) and starting (given after training loads – 2^nd group). On the entire surface of the transverse sections of the right lobe of the thyroid gland, after immunohistochemical staining with polyclonal antibodies to calcitonin, C-thyrocytes were analyzed using topographic and zonal principles. After qualitative description of C-cells (the position of cells or their associations to follicles: intra-, inter- and parafollicular) diameter was measured, their functional state was characterized with help of semiquantitative (the number of granules) and quantitative (cross-sectional area) methods.

Results. The average quantity of the C-cells, the space occupied by them and their quantity in groups at experimental animals were decreased (p<0.05), and at loadings of starting character there were quite often individual  C-cells. Calcitoninocytes were more often located about small follicles, probably, it has been connected with mechanisms of their passive moving in gland due to formation of new follicles.

Conclusions. It has been revealed, that adaptable changes of TG are closely connected with specific features and character of loads and lead to quantitative and qualitative structural exchanges of parafollicular cells, regulating a morphofunctional condition of the gland and its components.

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