Evaluation of microcirculation in tissues of skin regenerate using ultrasound dopplerography

Abstract

The aim is to study microcirculation in the skin tissues regenerate using Doppler ultrasound. Material and methods. In an experiment on laboratory white female rats, the effect of the angiovascular drug Neovasculgen on the repair of a full-thickness skin wound was studied. To assess the state of the microcirculatory bed of the regenerating granulation tissue of the regenerate, the ultrasound computerized device Minimax-Doppler-K was used. The processing dopplerograms obtained average systolic blood flow linear velocity, the mean systolic volumetric blood flow rate were calculated pulsation index, peripheral resistance. The data were processed by nonparametric statistics. Results. With the use of an angioactive drug, the volumetric flow velocity is increased, indicating a better blood supply in the area of the regenerating wound. The average systolic linear velocity of blood flow also increases, which is associated with vascular reduction. The indicators of the index of pulsation and peripheral resistance are reduced. Data on the linear and volume velocities of the blood flow made it possible to reveal elevated, in comparison with the control, indicators of the total cross-section of microvessels and the amount of blood entering the region of the regenerate under conditions of stimulated angiogenesis. Conclusion. Doppler examination allows to evaluate the course of all stages of the healing process, in the screening mode to monitor the microcirculatory bed in the regenerate area, to predict and correct the healing process.