Features of Connective Tissue’s Reparative Histogenesis in the Process of Eradication of an Infected, Rigid, Residual Cavity with Empyema of the Pleura

Abstract

The aim of the study was to study the features of reparative histogenesis in the process of eradication of the infected residual cavity with chronic empyema of the pleura using the filling material "LitAr". Material and methods. The experiment was carried out on 32 laboratory Wistar rats. Model of an aseptic restricted cavity in the right half of the thorax was created by implanting a latex ball into it. The creation of a model of limited chronic empyema of the pleura took up to 6 weeks. The infected cavity was filled at the stage of 40 days of the experiment only with a composite material. The resulting material was examined using survey histological methods (hematoxylin Mayer and eosin, and by Van-Geison’s method). The expression of the Ki-67 protein proliferation marker was determined in cut sections immunocytochemically. Results. The biodegradable "LitAr" composite stimulated angiogenesis, proliferation and cytodifferentiation of the cellular elements of fibroblastic diferon, the synthesis of components of the intercellular substance, which led to the formation of a loose, unformed connective tissue at the site of the cavity.