Features of Structural Changes in the Pyramidal Layer of a Hippocampus of Rats in Gravitational Overload in Caudo-Cranial Vector, Taking Into Account GFAP Expression
https://doi.org/10.18499/2225-7357-2017-6-2-75-82
Abstract
The aim of the study was to study the structural changes in the pyramidal layer of rats’ hippocampus under gravitational action. Material and methods. Adult rats were subjected to 28-day gravitational effects in the caudo-cranial vector for 5 minutes twice a day with an interval of 12 hours. Methods of thionin staining by Nissl and immunohistochemical detection of GFAP were used. The degree of immunopositive material expression was estimated by counting its specific area taking into account the distribution in areas of hippocampus. The specific number of damaged neurons, average areas of nuclei, cytoplasm and pericarion neurons, the specific density of neurons in the hippocampus were counted. Statistical processing of data is given in the form of a median (Me) with an interquartile interval [Q1; Q3]. Differences between the groups were assessed by the Mann-Whitney (U-test) criterion and considered statistically significant at p<0.05. Results. Microscopic examination revealed signs of circulatory disorders: stasis, congestion, swelling, degenerative changes of neurons in the form of wrinkling and hyperchromia of perikaryonic cytoplasm. A significant decrease of the specific density of neurons, a significant increase in the proportion the number of hyperchromic neurons, significant increase in GFAP expression in the pyramidal layer gliocytes CA2 and CA4 areas of the hippocampus. Conclusions. Gravitational action by the force of 9G leads to the activation of gliosis in the CA2 and CA4 areas of rats’ hippocampus in combination with the development of degenerative changes.
About the Authors
A. V. Smirnov
Volgograd Medical Research Center, Volgograd, Russia
Russian Federation
Russian Federation
M. V. Shmidt
Volgograd Medical Research Center, Volgograd, Russia
Russian Federation
Russian Federation
D. S. Mednikov
Volgograd State Medical University, Volgograd, Russia
Russian Federation
Russian Federation
M. R. Ekova
Volgograd State Medical University, Volgograd, Russia
Russian Federation
Russian Federation
I. N. Tyurenkov
Volgograd State Medical University, Volgograd, Russia
Russian Federation
Russian Federation
D. V. Kurkin
Volgograd State Medical University, Volgograd, Russia
Russian Federation
Russian Federation
E. V. Volotova
Volgograd State Medical University, Volgograd, Russia
Russian Federation
Russian Federation
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Review
For citations:
Smirnov A.V., Shmidt M.V., Mednikov D.S., Ekova M.R., Tyurenkov I.N., Kurkin D.V., Volotova E.V. Features of Structural Changes in the Pyramidal Layer of a Hippocampus of Rats in Gravitational Overload in Caudo-Cranial Vector, Taking Into Account GFAP Expression. Journal of Anatomy and Histopathology. 2017;6(2):75-82. (In Russ.) https://doi.org/10.18499/2225-7357-2017-6-2-75-82
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