Dynamics of the Morphofunctional State of Bone Marrow Cell-Differons as a Hematopoietic Organ

Abstract

The aim of the study is to evaluate the effect of bone marrow autotransplantation on its cell-differons. Material and methods. The study was performed on 40 white SJL male mice. In the 1st group there were 20 intact mice. Animals of the 2nd group (n=20) were injected with suspension of bone marrow taken from the femur of the same animal, in the caudal vein. The animals were removed from the experiment at 40 min and 2 h by decapitation. Mast cells were counted in 5 fields of view of the microscope. S. A. Cross, S. W. Even, F. W. Rost luminescent-histochemical method was used for tissue histamine detection. The degree of heparin sulphation and the state of mast cells were studied by A. Unna staining. Myelogram was counted in 500 cells after Pappenheim staining. Ki-67 was detected by MM-1 antibodies (NovoCastra, GB) according to standard protocols. For two group comparisons, Student's t-test was used. Differences were considered significant at p<0.05. Results. There is an increase in the number of mast cells, with an increased content of histamine. An increase in immune processes activity on the background of enhanced luminescence of lymphocytes and increase of cells mitotic activity. The number of young β-metachromatic mast cells is increasing. Ki-67 immunohistochemical staining revealed proliferating cells. Conclusions. Autogenous bone marrow transplantation promotes intensification of immune and proliferative processes in bone marrow structures of mice and also leads to the formation of young mast cells. Reduction of heparin sulfation in mast cells leads to histamine liberation and myelogram changes.