Karyometric Parameters of Dorsal Root Ganglia Neurons During Healing of Purulent Wounds

The aim was to conduct a karyometric assessment of neurons in dorsal root ganglia of experimental animals against the background of a purulent wound process with natural healing and with stimula- tion by hydropulse sanitation and the introduction of platelet-rich blood plasma. Material and methods. In an experiment on 100 white mongrel rats, a purulent wound of the lateral surface of the thigh was modeled by introducing a daily culture of Staphyllococcus aureus with a concentration of 1010 microbial bodies. Two groups were formed: natural healing and healing after therapy of hydro-pulse sanitation of the wound with a finely dispersed flow of NaCl followed by the introduction of platelet-rich autologous blood plasma. The material was taken on the 1st, 7th and 14th days. Excision of the lumbar spinal nodes of segments LIII–LV was performed as corresponding to the zonal innervation of the area of the inflicted wound. At the light-optical level, after staining with cresyl violet according to Nissl, the area of the central cross-section of the nuclei of nerve cells was measured, the number of multinucleolar neurons was counted. Statistical processing was carried out using nonparametric statistical methods Mann–Whitney test; for multiple group comparisons, Kruskal–Wallis ANOVA test was used. Results. The nuclei of neurons of dorsal root ganglia responded to the wound process in the area of their innervation by increasing their area, corresponding to the compensatory load against the background of an increase in the number of cells with signs of reactive changes. Conclusion. Combined therapy with hydropulse sanitation and platelet-rich autologous blood plasma leads to greater expression of compensatory reactions in all subpopulations of neurons, manifested in a statistically significant increase in the area of nuclei with maximum values on the 7th day of the experiment.

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