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Morphological and Functional Changes in Gum Tissues During Intoxication with Thiram Followed by Correction with Vitamin C and Silybum Extract

https://doi.org/10.18499/2225-7357-2023-12-3-65-71

Abstract

The widespread use of pesticides can lead to disruption of the ecological balance between the external environment and the human body, provoking the development of oxidative stress, which leads to an increase in toxic forms of damage to organs and systems of the macroorganism. The present study shows the effect of subchronic intoxication with the fungicide tiram on the morphological and functional state of gum tissues, as well as the use of plant antioxidants of vitamin C and silybum extract to stop the inflammatory process and  to increase  the regenerative potential of gum structural components.

The aim was to study of morphofuncmorphofunctional changes in gum tissue during subchronic intoxication with the fungicide tiram and correction with  vitamin  C  and  silybum  extract.

Material  and  methods.  The  experiment  was  performed  on  150  male Wistar rats, which were orally administered the fungicide thiram for 28 days, followed by antioxidant therapy. Morphofunctional changes in the mucosal epithelium and the cellular composition of the subepithelial layer were analyzed to assess the severity of inflammatory changes in histological preparations. Fibroblastic differon cells, macrophages and inflammatory cells of granulocytes and lymphocytes were differentiated by karyological signs, and the thickness of the epithelial layer, the areas occupied by the fibrous, vascular and cellular components were measured.

Results.  According  to  the  results  of  the  morphological  and  morphometric  analysis,  pathomorphological changes in the gum structure caused by pesticide intoxication were reliably proven. After the use of antioxidants,  there  was  a  significant  decrease  in  inflammatory processes  in  non-resident  cells,  the  number  of blood vessels in the lamina propria, as well as interstitial edema in it. Reparative processes were manifested by the migration of fibroblastic cells, the initiation of collagenogenesis, and an increase in the fibrous component.

Conclusion. Thus, the effect of using silybum extract and vitamin C as a means of normalizing the morphofunctional state of gum tissue after thiram intoxication has been shown.

About the Authors

E. S. Mishina
Kursk State Medical University
Russian Federation

Ekaterina S. Mishina – Cand. Sci. (Med.), Associate Professor of the Department of Histology, Embryology, Cytology

ul. Karla Marksa, 3, Kursk, 305041



V. A. Korolev
Kursk State Medical University
Russian Federation

Vladimir A. Korolev – Doct. Sci. (Biol.), Professor, Head of the Department of Biology, Medical Genetics and Ecology

Kursk



E. V. Fel'ker
Kursk State Medical University
Russian Federation

Elena V. Felker – Cand. Sci. (Med.), Associate Professor, Head of the Department of Orthopedic Dentistry

Kursk



M. V. Mnikhovich
A.P. Avtsyn Research Institute of Human Morphology of B.V. Petrovsky National Research Center of Surgery
Russian Federation

Maksim V. Mnikhovich – Cand. Sci. (Med.), leading researcher of Central pathoanatomical laboratory of Research Institute of Human Morphology

Moscow



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Review

For citations:


Mishina E.S., Korolev V.A., Fel'ker E.V., Mnikhovich M.V. Morphological and Functional Changes in Gum Tissues During Intoxication with Thiram Followed by Correction with Vitamin C and Silybum Extract. Journal of Anatomy and Histopathology. 2023;12(3):65-71. (In Russ.) https://doi.org/10.18499/2225-7357-2023-12-3-65-71

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ISSN 2225-7357 (Print)