Macrophages of the respiratory division of the lungs of rats after a single parenteral injection of silicon dioxide nanoparticles of various sizes
https://doi.org/10.18499/2225-7357-2022-11-3-114-117
Abstract
The aim of the study was macrophages analysis of the respiratory tract in rats after a single parenteral administration of the silicon dioxide nanoparticles in a size of 7 nm and 34 nm during 6 months.
Material and methods. The sections of the rat lung were studied using the methods of histology and immunohistochemistry after injection of 1 ml of 7 and 34 nm a SDNPs saline suspension at a concentration of 2 mg/ml (7mg/kg). Control animals were administered 1ml saline solution. To study chronic toxicity material sampling was performed on the 21st day, and 2, 4 and 6 months after the administration of the SDNPs (5 animals in the group) and fixed in 10% neutral formalin.
Results. An increase in the number of interstitial and alveolar macrophages were identified by 4 months the introduction of silicon dioxide nanoparticles with a size of 7 nm, with normalization of the indicators by 6 months. The use of 34 nm nanoparticles does not cause significant changes of the respiratory tract of rats at all times of the experiment.
Conclusion. A single parenteral administration of silica nanoparticles causes aseptic inflammation of the respiratory tract of rats and an increase in the number of alveolar macrophages by 4 months of the experiment only with the introduction of 7 nm silica nanoparticles. Both nanoparticle sizes don't cause tissue remodeling of the lung throughout the experiment.
About the Authors
G. Yu. Yukina
I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Galina Yu. Yukina – head of pathomorphological laboratory
ul. L'va Tolstogo 6/8, St. Petersburg, 197022
E. G. Sukhorukova
I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Elena G. Sukhorukova
St. Petersburg
I. V. Polovnikov
I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Il'ya V. Polovnikov
St. Petersburg
E. A. Kryzhanovskaya
I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Elena A. Kryzhanovskaya
St. Petersburg
S. G. Zhuravskii
I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Sergei G. Zhuravskii
St. Petersburg
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Review
For citations:
Yukina G.Yu., Sukhorukova E.G., Polovnikov I.V., Kryzhanovskaya E.A., Zhuravskii S.G. Macrophages of the respiratory division of the lungs of rats after a single parenteral injection of silicon dioxide nanoparticles of various sizes. Journal of Anatomy and Histopathology. 2022;11(3):114-117. (In Russ.) https://doi.org/10.18499/2225-7357-2022-11-3-114-117
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