Expression of 5-HT1A receptors in skin of rats under dark deprivation
https://doi.org/10.18499/2225-7357-2022-11-3-57-64
Abstract
The aim of the investigation was to study the dynamics of serotonin 5-HT1A receptor expression in the skin of white male rats under dark deprivation.
Material and methods. 20 outbred male rats 170–220 grams by mass were used in the experiment. The animals were divided into 2 groups. The intact group included animals under standard fixed lighting conditions (12 h light / 12 h dark). The experimental group consisted of animals with simulation of dark deprivation under conditions of round-the-clock illumination (24 h light). The duration of the study was 21 days. The object of the study was the skin of rats. Using immunohistochemistry, the features of the expression of 5-HT1A receptors were studied.
Results. A strong circadian dependence of the expression of serotonin 5-HT1A receptors in the general integument has been established. In the cells of the epidermis, sebaceous glands and hair follicles throughout the experiment, a decrease in the proportion of immunopositive cells was observed with a simultaneous increase in the positivity of 5-HT1A expression.
Conclusion. Throughout the experiment, a strong circadian dependence of the expression of serotonin 5-HTR1A receptors in the general integument was expressed, which, apparently, determines the mechanism of an adequate response to the destabilization of circadian rhythms.
About the Authors
I. S. Sobolevskaya
Vitebsk State Medical University
Belarus
Belarus
Irina S. Sobolevskaya – Cand. Biol. Sci., Assoc. Prof. of department of histology, cytology and embryology
prospekt Frunze, 27, Vitebsk, 210009
M. I. Krasnobaeva
Vitebsk State Medical University
Belarus
Belarus
Marina I. Krasnobaeva – postgraduate student
Vitebsk
O. D. Myadelets
Vitebsk State Medical University
Belarus
Belarus
Oleg D. Myadelets – Doct. Med. Sci., Professor
Vitebsk
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Review
For citations:
Sobolevskaya I.S., Krasnobaeva M.I., Myadelets O.D. Expression of 5-HT1A receptors in skin of rats under dark deprivation. Journal of Anatomy and Histopathology. 2022;11(3):57-64. (In Russ.) https://doi.org/10.18499/2225-7357-2022-11-3-57-64
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