anatomyЖурнал анатомии и гистопатологииJournal of Anatomy and Histopathology2225-7357N.N. Burdenko Voronezh State Medical University10.18499/2225-7357-2019-8-3-89-95anatomy-961Research ArticleМЕТОДИКИ ИССЛЕДОВАНИЯПрименение программы анализа изображений для определения формы просветов биологических объектов на примере желез простаты человекаImage Analysis Program Application for Determining the Shape of Lumens of Biological Objects on the Example of Human Prostate GlandsПетькоИ. А.Pet'koI. A.

Петько Ирина Александровна

пр-т Фрунзе, 27, Витебск, 210009

Irina Pet'ko

pr-t Frunze, 27, Vitebsk, 210009

usovicha@mail.ruУсовичА. K.UsovichA. K.Витебский государственный ордена Дружбы народов медицинский университетVitebsk State Order of Peoples’ Friendship Medical University201915102019838995Copyright © Петько И.А., Усович А.K., 20192019Петько И.А., Усович А.K.Pet'ko I.A., Usovich A.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://anatomy.elpub.ru/jour/article/view/961

Цель работы – оценить информативность применения компьютерных программ анализа изображений для определения формы просветов биологических объектов на примере возрастных изменений желез простаты человека в разных структурных дольках органа.

Материал и методы

Материал и методы. Исследовано 37 простат мужчин в возрасте от 17 до 75 лет, с применением программы открытого доступа «ImageFiji». Определение формы просветов концевых отделов желез проводилось на гистологических препаратах, окрашенных гематоксилином и эозином, фукселином по Hart и азокармином по Heidenhain, в переднемедиальной, верхнемедиальной, нижнезадней, нижнебоковой дольках правой и левой половин простаты. Для описания формы просветов концевых отделов желез простаты использовали диаметр Фере и «характеристики формы», включающие следующие факторы формы: округлость, индекс закругленности, индекс компактности. Статистическая обработка полученных данных выполнена при помощи пакетов прикладных статистических программ «Microsoft Excel’2007» и Statistica 10».

Результаты

Результаты. Обнаружили, что форма просветов концевых отделов желез в каждой из структурных долек простаты с возрастом изменяется не одинаково.

Заключение

Заключение. Предложенный способ количественного определения изменений формы просветов биологических объектов, основанный на использовании возможностей компьютерной программы анализа изображений ImageFiji, информативен при оценке их изменений. Полученные результаты способствуют расширению имеющихся в настоящее время научных представлений о возрастных преобразованиях желез простаты и могут использоваться как при исследовании возрастных, половых, индивидуальных особенностей строения структур человека и животных, так и на этапе предварительной оценки наличия патологических изменений.

The aim of study was to evaluate the informative value of computer-based image analysis programs for determining the shape of lumens of biological objects investigating age-related changes in the human prostate glands in various structural lobules of the organ as an example.

Material and methods

Material and methods. The study included 37 men aged 17-75, whose prostates were examined using the open-access program “ImageFiji”. Histological slides stained with hematoxylin and eosin, fuchselin with Hart’s modification and azocarmine according to Heidenhain were used to determine the shape of lumens of adenomeres in the anteromedial, upper medial, inferior, lower lateral lobes of the right and left halves of the prostate. The Feret diameter and “shape characteristics” were used to describe the shape of the lumens of prostate adenomeres; this including the following shape factors: roundness, roundness index, and compactness index. The obtained data were statistically processed using “Microsoft Excel’2007” and “Statistica 10” programs.

Results

Results. The results obtained demonstrated that the shape of the lumens of adenomeres in each of the structural lobes of the prostate does not vary in the same manner with age.

Conclusion

Conclusion. The proposed method for the quantitative determination of changes in the shape of lumens of biological objects based on the potentials of “ImageFiji”, a computer program for analyzing images, is considered to have an informative value when evaluating these changes. The obtained results contribute to the expansion of the current scientific understanding of age-related transformations of the prostate glands and can be used both in the study of age, sex, individual structural features of human and animal structures, and at the stage of preliminary assessment of the presence of pathological changes.

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The authors declare that there are no conflicts of interest present.