anatomyЖурнал анатомии и гистопатологииJournal of Anatomy and Histopathology2225-7357N.N. Burdenko Voronezh State Medical University10.18499/2225-7357-2017-6-3-19-24anatomy-357Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL PAPERSВОЗРАСТНЫЕ ОСОБЕННОСТИ ЭКСПРЕССИИ ДАБЛКОРТИНА В СТРУКТУРАХ ОБОНЯТЕЛЬНЫХ ЛУКОВИЦ КРЫСЫAge-Related Differences of Doublecortin Expression in the Rat’s Olfactory BulbsВаренцовВячеслав ЕвгеньевичVarentsovV. E.carabidolog@mail.ruРумянцеваТатьяна АнатольевнаRumyantsevaT. A.rum-yar@mail.ruФГБОУ ВО «Ярославский государственный медицинский университет» Минздрава РоссииYaroslavl State Medical University, Yaroslavl, Russia201728122017631924Copyright © Варенцов В.Е., Румянцева Т.А., 20172017Варенцов В.Е., Румянцева Т.А.Varentsov V.E., Rumyantseva T.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://anatomy.elpub.ru/jour/article/view/357

Цель исследования - изучить возрастные морфологические характеристики обонятельных луковиц по распределению даблкортина (DCX) у крыс от 3 до 180 суток жизни. Материал и методы. DCX, маркер иммунореактивных нейрональных предшественников, выявляли на парасагиттальных срезах с учетом слоев, определяли численную плотность тел DCX-позитивных клеток (шт/мм2) и их площадь (мкм2). Результаты. Активность DCX отмечалась в телах и отростках части клеток, плотность распределения зависела от слоя луковицы и возраста животных. У новорожденных, плотность позитивных нейронов в слоях луковиц не велика, затем нарастала, достигая максимума в митральном слое на 7-е, а в гранулярном - на 14-е сутки жизни. У крыс 2-месячного возраста плотность нейронов снижалась, но по-прежнему превышала показатели новорожденных, только у шестимесячных крыс плотность DCX+ клеток снижалась до минимума. Выводы. Снижение активности нейрогенеза по экспрессии даблкортина в обонятельной луковице у крыс происходило только к периоду полового созревания. Увеличение DCX иммунореактивности в периоде новорожденности, вероятно, обусловлено перинатальным стрессом, процессами адаптации к внеутробным условиям существования, расширением спектра и интенсивности сенсорных стимулов.

The aim was to study age characteristics of doublecortin (DCX) distribution in olfactory bulbs of rats ranging from 3 to 180 days of life. Material and methods. DCX, a marker of immunoreactive neuronal precursors, was detected on parasagittal sections with distribution for layers. The numerical density of the positive cells bodies (pcs/mm2) and their sizes were determined. Results. It was found that the DCX activity is noted in the bodies and in the processes of a part of the cells. The distribution density depends on the bulb layer and on the animal’s age. In newborns the density of positive neurons in the bulbs layers is small, then it increases, reaching a maximum in the mitral layer by 7th and in the granular layer - by the 14th day. In 2-month aged rats the density of neurons decreases but still exceeds the rates of newborns. The density of DCX+ cells falls to the minimum only in six-month rats. Thus, a decrease in the activity of neurogenesis according to the expression of doublecortin in the olfactory bulb in rats occurs only to the period of puberty.

обонятельные луковицынейрогенездаблкортинонтогенезмаркернейробластростральный миграционный потокolfactory bulbsneurogenesisdoublecortinontogenesismarkerneuroblastrostral migratory streamReferencesГомазков О. А. Нейрогенез как адаптивная функция мозга. М.; 2014. 85.Гомазков О. А. Нейрогенез как адаптивная функция мозга. М.; 2014. 85.Balu D. T. Adult hippocampal neurogenesis: regulation, functional implications, and contribution to disease pathology. J. Neurosci Biobehav. 2009; Rev 33 (3): 232-252.Balu D. T. Adult hippocampal neurogenesis: regulation, functional implications, and contribution to disease pathology. J. Neurosci Biobehav. 2009; Rev 33 (3): 232-252.Brown J. P., Couillard-Després S., Cooper-Kuhn C. M., et al. Transient expression of doublecortin during adult neurogenesis. J. Comp. Neurol. 2003; 467 (1): 1-10.Brown J. P., Couillard-Després S., Cooper-Kuhn C. M., et al. Transient expression of doublecortin during adult neurogenesis. J. Comp. Neurol. 2003; 467 (1): 1-10.Couillard-Despres S., Winner B., Schaubeck S., et al. Doublecortin expression levels in adult brain reflect neurogenesis. J. Neurosci. 2005; 21 (1): 1-14.Couillard-Despres S., Winner B., Schaubeck S., et al. Doublecortin expression levels in adult brain reflect neurogenesis. J. Neurosci. 2005; 21 (1): 1-14.Faiz M., Acarin L., Castellano B., Gonzalez B. Proliferation dynamics of germinative zone cells in the intact and excitotoxically lesioned postnatal rat brain. J. BMC Neuroscience. 2005; 6(26): 1-16.Faiz M., Acarin L., Castellano B., Gonzalez B. Proliferation dynamics of germinative zone cells in the intact and excitotoxically lesioned postnatal rat brain. J. BMC Neuroscience. 2005; 6(26): 1-16.Jung H. C., Jung H. C., Ki Y. Y., et al. Comparison of newly generated doublecortin-immunoreactive neuronal progenitors in the main olfactory bulb among variously aged Gerbils. J. Lab. Anim. Res. 2010; 26(1): 121-125.Jung H. C., Jung H. C., Ki Y. Y., et al. Comparison of newly generated doublecortin-immunoreactive neuronal progenitors in the main olfactory bulb among variously aged Gerbils. J. Lab. Anim. Res. 2010; 26(1): 121-125.Lucassen P. J. Regulation of adult neurogenesis by stress, sleep disruption, exercise and inflammation: Implications for depression and antidepressant action. J. Eur Neuropsychopharmacol. 2010; 20 (1): 1-17.Lucassen P. J. Regulation of adult neurogenesis by stress, sleep disruption, exercise and inflammation: Implications for depression and antidepressant action. J. Eur Neuropsychopharmacol. 2010; 20 (1): 1-17.Marchis S., Bovetti S., Carletti B., et al. Generation of distinct types of periglomerular ofactory bulb interneurons during development and in adult mice: implication for intrinsic properties of the subventricular zone progenitor population. J. Neuroscience. 2007; 27(3): 657-664.Marchis S., Bovetti S., Carletti B., et al. Generation of distinct types of periglomerular ofactory bulb interneurons during development and in adult mice: implication for intrinsic properties of the subventricular zone progenitor population. J. Neuroscience. 2007; 27(3): 657-664.Meerlo P. New neurons in the adult brain: the role of sleep and consequences of sleep loss. J. Sleep Med Rev. 2008; 13(3): 187-194.Meerlo P. New neurons in the adult brain: the role of sleep and consequences of sleep loss. J. Sleep Med Rev. 2008; 13(3): 187-194.Romero-Grimaldi C., Moreno-López B., Estrada C. Age-dependent effect of nitric oxide on subventricular zone and olfactory bulb neural precursor proliferation. J. Comp Neurol. 2008; 506 (2): 339-346.Romero-Grimaldi C., Moreno-López B., Estrada C. Age-dependent effect of nitric oxide on subventricular zone and olfactory bulb neural precursor proliferation. J. Comp Neurol. 2008; 506 (2): 339-346.Wille M., Schümann A., Kreutzer M., et al. The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study. J. Proteome Science. 2015; 13 (8).Wille M., Schümann A., Kreutzer M., et al. The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study. J. Proteome Science. 2015; 13 (8).Yang Z. Postnatal subventricular zone progenitors give rise not only to granular and periglomerular interneurons but also to interneurons in the external plexiform layer of the rat olfactory bulb. J. Comp. Neurol. 2008; 506 (2): 347-358.Yang Z. Postnatal subventricular zone progenitors give rise not only to granular and periglomerular interneurons but also to interneurons in the external plexiform layer of the rat olfactory bulb. J. Comp. Neurol. 2008; 506 (2): 347-358.

The authors declare that there are no conflicts of interest present.