anatomyЖурнал анатомии и гистопатологииJournal of Anatomy and Histopathology2225-7357N.N. Burdenko Voronezh State Medical University10.18499/2225-7357-2020-9-3-94-101anatomy-1162Research ArticleМЕТОДИКИ ИССЛЕДОВАНИЯНовый гистохимический подход для оценки экспрессии триптазы в популяции тучных клетокNovel histochemical approach for evaluation of tryptase expression in the mast cell populationАтякшинД. А.AtyakshinD. A.

ул. Студенческая, 10, Воронеж, 394036, Российская Федерация

ul. Studencheskaya, 10, Voronezh, 394036, Russian Federation

earth-mars38@yandex.ruГерасимоваО. А.GerasimovaO. A.

Воронеж

Voronezh

МешковаВ. Ю.MeshkovaV. Yu

Воронеж

Voronezh

СамодуроваН. Ю.SamodurovaN. Yu.

Воронеж

Voronezh

СамойленкоТ. В.SamoilenkoT. V.

Воронеж

Voronezh

ШишкинаВ. В.ShishkinaV. V.

Воронеж

Voronezh

ФГБОУ ВО «Воронежский государственный медицинский университет имени Н.Н. Бурденко» Минздрава РоссииN.N. Burdenko Voronezh State Medical University2020101020209394101Copyright © Атякшин Д.А., Герасимова О.А., Мешкова В.Ю., Самодурова Н.Ю., Самойленко Т.В., Шишкина В.В., 20202020Атякшин Д.А., Герасимова О.А., Мешкова В.Ю., Самодурова Н.Ю., Самойленко Т.В., Шишкина В.В.Atyakshin D.A., Gerasimova O.A., Meshkova V.Y., Samodurova N.Y., Samoilenko T.V., Shishkina V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://anatomy.elpub.ru/jour/article/view/1162

Для изучения селективного влияния тучных клеток на состояние специфического тканевого микроокружения необходима детализация молекулярного состава их секретома и анализ путей дегрануляции. Разработанный метод сочетания иммуноморфологического и гистохимического протоколов окрашивания триптазы способствует более объективному определению интегрального уровня экспрессии специфической протеазы в популяции тучных клеток кожи. Дополнительные возможности визуализации цитологических характеристик и особенностей процессинга триптазы расширяют эффективность морфологического анализа как в норме, так и при патологии.

The paper presents a novel histochemical approach for evaluation of tryptase expression in the mast cell population. To study the selective effect of mast cells (MC) on the parameters of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. The developed method for combined immunomorphological and histochemical tryptase staining protocols contributes to a more objective determination of the integral level of specific protease expression in the skin MC population. Extra visualization potentials of cytological features and specific aspects of tryptase processing expand efficiency of morphological analysis in both normal and pathological conditions.

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The authors declare that there are no conflicts of interest present.